240 IVAN V. SHUNK 



Harrison and Barlow (1907) reported a single polar flagellum 

 on the organisms from Lathy rus sativus and Vicia villosa but 

 their method of staining was such that only the slime and muci- 

 lage around the bacteria were stained, leaving the bacteria 

 themselves unstained. 



Zipfel (1912) reported numerous peritrichic flagella, but did 

 not state the source of his organisms. 



Kellerman (1912) reported numerous peritrichic flagella on 

 the organisms from Phaseolus lunatus, Medicago saliva and Pisum 

 sativum. 



Burrill and Hansen (1917) have given us the most extensive 

 information on the question of the flagellation of this group. 

 They reported a single flagellum on organisms from Vigna sinensis^ 

 Glycine hispida, Acacia floribunda, Arachis hypogoea, Baplisia 

 tinctoria, Genista tinctoria, Cassia chaemacrista, Amphicarpa 

 monoica, Lespedeza striata, Desmodiuni canescens, and Mucuna 

 utilis. 



Fred (1918) has found on the alfalfa organism several peri- 

 trichic flagella and on the lupine organism one or rarely two. 



Prucha (1915) has found several flagella on the organism from 

 the Canada field pea. 



Wilson (1917) reported as many as four flagella on the soy 

 bean organism. 



Hansen (1919) has found peritrichic flagella on the organisms 

 from Trifolium pratense, Vicia villosa, and Melilotus alba. 



Much of the difficulty in staining the flagella of these bacteria 

 has been and still is due to the amount of slime that is produced 

 by these organisms. Some produce slime more abundantly 

 than others, and in some cases the mass of bacteria is so viscid 

 that it may be drawn out in a string in making transfers. 



METHOD OF ISOLATION 



The roots of various leguminous plants were dug and carefully 

 washed in running tap water. Small pieces of roots bearing 

 nodules were then removed leaving enough root attached to aid 

 in handling. These nodules were allowed to stand in tap water 



