FLAGELLATION OF BACTERIA OF LEGUMINOSAE 245 



3. As suggested by Hansen (1919) the writer believes that the 

 nodule bacteria of the Leguminosae are of two groups and if we 

 follow Migula's classification they belong to two genera, Pseudo- 

 monas and Bacillus. 



4. From 15 genera the flagella were of the single flagellate 

 type. From 8 genera the flagella were of the peritrichic type. 



5. In no case has anj^ difference been found in the type of 

 flagellation on organisms from plants of different species of a 

 genus. 



6. The single flagellate type is not strictly polar as the flagel- 

 lum is usually attached at the corner rather than exactly at 

 the end. 



ACKNOWLEDGMENTS 



The writer desires to express his thanks and appreciation to 

 Dr. F. A. WoK for kindly advice and assistance throughout the 

 work. 



REFERENCES 



BuRRiL, T. J., AND Hansen, R. 1917 Is symbiosis possible between legume 

 bacteria and non-legume plants? 111. Agr. Exp. Sta. Bui. 202, 122-123, 

 136-137, plates III, IV, V. 



Conn, H. J., and Breed, R. S. 1920 A suggestion as to the flagellation of the 

 organisms causing legume nodules. Sci. N. S., 51, 391-392. 



De Rossi, Gino 1907 Ueber die Mikroorganismen, welche die Wurzelknollchen 

 der Leguminosen erzeugen. Centralb. f. Bakt., 2 Abt., 18, 304. 



De Rossi, Gino 1920 Studien iiber den knollchenerzeugenden Mikroorganis- 

 mus der Leguminosen. I. Isolierung, bacteriologische Diagnose, 

 Anwendbarkeit der Kulturen in der landwirtschaftlichen Praxis. 

 Centralb f. Bakt., 2 Abt., 26, 267. 



Fred, E. B., and Davenport, A. 1918 Influence of reaction on nitrogen assim- 

 ilating bacteria. Jour. Agr. Res., 14, 320-321. 



Garman, H., and Didlake, Mary 1914 Six different species of nodule bacteria. 

 Ky. Agr. Exp. Sta. Bui. 184, 343-344. 



Hansen, R. 1919 Note on the flagellation of the nodule organisms of the 

 Leguminosae. Sci. N. S., 50, 568-569. 



Harrison, F. C, and Barlow, E. 1907 The nodule organism of the Legumi- 

 nosae — its isolation, cultivation, identification, and commercial appli- 

 cation. Centralb. f. Bakt., 2 Abt., 19, 427-428, Taf. II, 9, III, 14. 



Kellerman, K. F. 1912 The present status of soil inoculation. Centralb. 

 f. Bakt., 2 Abt., 34, 42-46, Taf. II. 



Prucha, M. J. 1915 Physiological studies of Bacillus radicicola of Canada 

 field pea. Cornell Agr. Exp. Sta. Mem. 5, 16-18. 



Shtjnk, I. V. 1920 A modification of Loeffler's flagella stain. Jour. Bact., 5, 

 181-187. 



