282 KAN-ICHIRO MORISHIMA 



fails to form indol or liquefy gelatin, will not grow in asparagin- 

 mannitol medium, does not reduce neutral red and causes brown- 

 ing of lead acetate media. It has low tolerance for acid, but 

 rather high tolerance for brilliant green dyes and alkali. It 

 has characteristic serum agglutination reactions and is found in 

 human stools and urines as an actual or potential cause of typhoid 

 fever. 



OUR OWN WORK ON VARIATION IN THE UTILIZATION OF 

 CARBOHYDRATES 



Some of this work has already been reported in the Journal 

 of Infectious Diseases, 1920, 26, 52-76. The following table 

 represents a condensed summary of previous work, together with 

 new experiments performed since then : 



Sugar fermentation 

 Arabinose broth 



Number of 

 strains 



First series. Meat infusion broth (litmus indicator) 114 



Second series. Nutrose broth (phenol red, china blue, indicators). 117 



Third series. The same medium 21 



Fourth series. Meat infusion broth (phenol red, china blue, indi- 

 cators) 24 



Results were as follows: 



Positive on the second day '. 2 



Positive on the third day 1 



Positive on the fourth day 2 



Positive on the sixth day 6 



Positive on the seventh day 4 



Positive on the ninth day 1 



Positive on the tenth day 2 



Positive on the eleventh day 1 



Positive on the fourteenth day 1 



Positive on the twenty-third day 1 



Positive on the twenty-fourth day 1 



Positive on the twenty-eighth day 1 



Positive (total) 23 



Negative on the 30th day 279 



The proportion of positives is 8.24 per cent against 91.76 per cent of negatives. 



