284 KAN-ICHIRO MORISHIMA 



Glycerol 



Glycerol broth 



We prepared 1 per cent glycerol meat infusion broth, using 

 china blue-phenol red indicator. 



strains 



First series 123 



Second series 10 



Third series 10 



Fourth series 10 



Total 153 



All of the cultures produced acidity. 



Recently we studied these phenomena with 257 freshly isolated 

 typhoid cultures from typhoid carriers' stools, using two different 

 percentages of glycerol, namely 2 per cent and 6 per cent. We 

 never found any alkali production in the early stage of incubation, 

 but observed acid production between the third and eighth 

 day; later some of the cultures showed reduction of color. 



Inosite 



Inosite is not a true sugar, but its character is similar to that 

 of sugar and therefore it deserves discussion here. 



Inosite broth 



One hundred and forty-two strains were inoculated in 1 per 

 cent inosite meat infusion broth, using china blue, phenol red 

 indicator. No fermentation or gas production resulted after 

 thirty days' incubation. Nine subcultures were transferred from 

 inosite broth to inosite broth with the same result. 



Raffinose 

 Raffinose broth 



We used 1 per cent raffinose meat infusion broth, with china 

 blue-phenol red indicator. In the first and fourth series, typhoid 

 bacilli did not grow. The media showed shght acidity before 



