288 KAN-ICHIRO MORISHIMA 



(b) Dulcitol 



Aerobic : On tenth day, two cultures showed good acidity and on 

 twentieth day four of them showed good acidity. 



Anaerobic: Did not show acidity on tenth day; on twentieth day 

 three of them showed sHght acidity. 



(c) Rhamjiose 



Aerobic : On the fourth day showed good alkahnity. 

 Anaerobic : On fifteenth day began to show alkalinity. 



(d) Xylose. 



True xylose fermenters ferment in twenty-four hours, under 

 both conditions. The results with four slow fermenters follow: 

 Aerobic: On sixth day one of them and on the eighth day two 



strains began to ferment. 

 Anaerobic: Up to the twentieth day no change in reaction 



appeared, then the same strains that fermented xylose under 



aerobic conditions produced very slight acidity. 



(e) Arahinose 



Aerobic: On the third day, they showed alkalinity and on the 



fifth day one strain show^ed slight acidity. 

 Anaerobic: Slight alkalinity persisted until the twenty-first day. 



(f) Raffinose 



Aerobic: On second day showed alkalinity. 



Anaerobic: On the eighteenth day showed slight alkalinity. In 

 second series, we tested four strains, and in a third series, 

 four strains on xylose broth but they did not show sufficiently 

 definite changes to be described here. 



It will be seen from the above that our own work does not 

 in every way agree with the work of others cited at the begin- 

 ning of this section. As stated above, we believe that the strains 

 of Klotz, McNaught and Wilson cannot be definitely accepted 

 as having been true typhoid bacilli. 



In regard to Mandelbaum's Bacillus metatyphi we feel that 

 our results seem to indicate that Mandelbaum was dealing with 

 slow glycerol fermenters rather than with known fermenting 

 strains. We should add, however, that we ourselves never 

 encountered any strains which exhibited the characteristics 

 described by Mandelbaum. 



As for the strains of Jacobsen none of our cultures corresponded 

 to these. 



