VARIATIONS IN TYPHOID BACILLI 



303 



the other sera "0-51" and "3," for agglutination tests, but did 

 not observe any striking difference between Rawling's seruni 

 and the other two sera in agghitinating Rawling's strain. 



2. "C-188" strain was tested in the same way as the Rawling's 

 strain. 



Cnart I 



Rawling strain against Rawling's serum antityphoid 



Dilution of serum 

 20,000 

 10,000 

 8,000 

 6,000 

 3,000 

 2,000 

 1,000 



900 \ 



800 '» f--' 



600 ". /. : 



KQO V ' : 



300 V 



100 

 50 

 



Day of agglutination test at3 6 1024273338 



once 

 NumTDer of transplants 2 5 9 15162224- 



MarKs 



-• Control culture plain troth culture 



* 24- hours reading ") 

 « \— . _ \ ^ ^ > serurn jnedia culture 



» - 2 hours Incuhatlon 



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In this case, we made agglutination tests eleven times within 

 sixty-three days. Its agglutinability fell from 1 : 8000 to 1 : 3000 

 after one day's cultivation in serum media. Three days later 

 agglutination occurred only in dilution of 1 : 100. Six days later, 



