304 



KAN-ICHIRO MORISHIMA 



it was below 1 :50. On the twenty-seventh day it rose to 1 :300 

 and after the thirty-fifth day to 1 : 20,000. Thereafter, from the 

 thirty-fifth to the sixty-third day, which was the last day of this 

 experiment, the serum strain and the broth control remained 

 entirely parallel. Other sera, C-51 and 3, were frequently used 

 after the twenty-third day but no differences were seen between 

 Rawling's serum and the other two sera. 



Chart II 

 oo.nen strain agglutination against Rawllng's antltypnold serum 



Dilution of serum 



20,000 



10,000 



8,000 



6,000 



3,000 



2,000 



1,000 



900 



800 



600 



4.00 



300 



100 



50 







Days of agglutination test at 1 3 6 lQ232i|2733384-7ii9636U6671737785 



once 

 Times transplanted on media 1 2 $ 9131516222J|283031323i*3639WJf3 



2H Hours reading, control plain trota culture 



2 Hours reading "^ 



2U Hours readlngj^^^ ^^'^^ culture 



3. Cohen strain was tested in the same manner. We tested 

 its agglutinability twenty-three times within eighty-five days, 

 within which period we made forty-three transplantations. The 

 results are shown on the next chart. 



After seventy-one days this strain recovered its agglutinabihty 

 gradually and at seventy-seven days it had almost completely 

 recovered this property. But readings two hours after incu- 

 bation were always slightly lower than the twenty-four hours' 

 readings. On the fourth day very sUght agglutination in dilu- 



