306 KAN-ICHIRO MORISHIMA 



Agglutination tests of each culture were carried out five times 

 within forty-three days, using Rawling's serum for the tests. 

 Twenty-eight transplants were made during this period. 



III. Since the experiments done so far seemed to show a 

 delicate difference between the reactions of individual strains 

 with different antityphoid sera, we decided to repeat them carry- 

 ing a single typhoid strain both on an homologous serum and 

 on an antityphoid serum produced with another stock strain. 

 For this purpose we immunized two rabbits, in one case using 

 no. 3 culture and in the other C-51. The former is a rapid 

 xylose fermenter and the latter a slow xylose fermenter. We 

 obtained sera which agglutinated our laboratory strains and their 

 own specific strains in dilution of 1:15,000. Cultivation in 

 normal sermn in no case changed the agglutinability of the 

 bacilli, which remained parallel in every way to that of the 

 cultures carried on plain broth. Serum media was again pre- 

 pared (one part serum to four parts broth) and tubed in small 

 tubes. Experiments as described above were then carried out. 



(1) Strain C-51. After two days' cultivation both in its own 

 serum broth and in no. 3 serum broth, this strain sUghtly lost 

 in agglutinability (1:8000). This continued for twelve days, 

 but on the fifteenth day both series recovered agglutinability 

 equal to that shown by the control cultures. 



In no. 3 serum broth its agglutinability was lowered sHghtly 

 more than by growth in the homologous C-51 serum broth, 

 but there was no very considerable difference between them. 



(2) Strain no. 3. This strain was tested in the same way as 

 C-51, by cultivation in its own serum broth and in C-51 serum 

 broth. On the third and seventh days inagglutinability on serum 

 no. 3 was most marked (1:100) both for the culture carried on 

 no. 3 serum and for the one carried on C-51 serum. Both 

 gradually recovered agglutinability and reached normal agglutin- 

 abiUty on the thirty-sixth day. 



When we used C-51 serum for agglutination tests similar 

 results were obtained. On the third day both series reached 

 the maximum point (1:2000) of inagglutinability. Then they 

 gradually recovered, although in this case recovery to normal 

 agglutinability was delayed for twenty-two days. 



