308 KAN-ICHIRO MORISHIMA 



easy to break up the spongelike growth. A few days later the 

 broth becomes turbid, at first, with a few clumps of agglutinated 

 bacteria, and later only a uniform turbidity is present; in the 

 last stage, at least one month later, the cultures grow slightly 

 more turbid in the tubes, and about two months later, the general 

 turbidity of the cultures is only slightly less marked than that 

 of control cultures in broth. 



The experiments just described w^ere carried out in concen- 

 trated antityphoid serum media. It seemed important to 

 observe what influence would be exerted on typhoid bacilli by 

 cultivation in broth which contained a very low percentage of 

 antityphoid serum. This might perhaps tell us what influence 

 would be exerted upon the bacilli in the early stages of typhoid 

 fever. 



We prepared a number of broth tubes containing Rawling's 

 serum in a proportion of 1:10,000. The titre of this serum 

 against Rawling's culture was 1 : 10,000. We used two strains 

 for these observations, one the Rawling's strain, the other a 

 culture designated as Sanguist, which had been freshly isolated 

 from a patient's blood. 



We carried out agglutination tests eight times within thirty- 

 three days during which we made twenty-two transplants. 

 Rawling's strain showed practically no change, a result distinctly 

 in contrast with its loss of agglutinability when grown in con- 

 centrated serum broth. The Sanguist strain diminished in 

 agglutinability after three days although the difference between 

 the serum culture and the control broth culture was not great. 

 After thirty-two transplants thirty-three days later, it regained 

 agglutinability completely. In short, in this experiment a 

 small amount of antityphoid serum in media did not produce 

 any marked change in agglutinability of typhoid bacilli. 



When the serum contained blood cells the typhoid bacilli 

 acted haemolytically and reduced the medium from a reddish 

 color to yellow in twenty-four hours. 



Several varieties of sugar media and litmus milk were inocu- 

 lated from the cultures described above and no differences 

 appeared between control cultures and serum broth cultures 

 within three weeks. 



