310 



KAN-ICHIRO MORISHIMA 



It is worth noting in this connection that Lieut.-Col. H. J. 

 Nichols tells us that he has found no difference between rapid 

 and slow xylose fermenters in regard to their virulence for 

 rabbits. 



Having determined the peculiar conditions under which 

 typhoid bacilli become inagglutinable and subsequently regain 

 their agglutinabihty, without removal from the specific immune 

 serum, it seemed important to determine whether this was due 

 to inability to absorb the agglutinins or possibly whether it had 

 some relationship to a changed reaction to electrolytes in solu- 

 tion. Also it seemed important to determine whether or not 

 something analogous to an insulating capsule as described by 

 Bail, Kuhnemann and others was responsible for the phenomenon. 



Accordingly we first proceeded to carry out agglutinin absorp- 

 tion tests as follows : 



a. Agglutinin absorption test 



This test was carried out several times using the Cohen strain 

 culture in serum broth for sixty-six days with 49 transplants in 

 senmi broth (see chart 2). As controls Cohen and C-188 strains 

 were cultures in plain broth. The serum used was usually 

 Rawling's, sometimes others. The technique employed was that 

 described in the section on agglutinin absorption tests of xylose 

 quick fermenter and xylose slow fermenter. The results follow : 



4--1--I- = complete agglutination, 

 -f -H = good agglutination. 

 + = good agglutination but still cloudy. 

 — = negative. No agglutination. 



