322 KAN-ICHIRO MORISHIMA 



Finally, we believe that since all the alterations brought about 

 by artificial en\'ironment in the tjiDhoid bacillus were rapidly 

 lost when the organisms were returned to the en\dromnents pre- 

 vailing under the usual cultural conditions and in the case of the 

 inagglutinable strains, even in the course of persistent abnormal 

 environment, the changes observed by others as well as by us 

 should properly be regarded as variants and cannot be spoken 

 of with accuracy as mutations in the sense of de Vries. 



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