STUDIES ON AZOTOBACTER CHROOCOCCUM BEIJ. 



347 



From these data it seems evident that the stores which were 

 accumulated by the cells during the first twenty-four hours of 

 development on a complete medium were subsequently attacked 

 when no more sugar was at their disposal. Nevertheless it must 

 be stated that the granules may not be the only stores of the 

 Azotobacter cell, and it is very probable that the heavy gelat- 

 inous capsule described by various authors is also a storing as 

 well as a protecting organ. 



TABLE 12 

 Autophagy of Azotobacter 



TABLE 13 

 Autophagy of Azotobacter 



Sugar: cells ratio 



Among the assimilation products of Azotobacter we should also 

 consider such compounds as play only a transitory role in the 

 cell metabolism and are later secreted in the surrounding medium. 

 Stoklasa believes these to be ethyl alcohol, lactic, acetic and 

 butyric acids, but we have seen that we should question his 

 results since we have reason to accept Krzmieniewsky's data and 

 Omeliansky's statements with regard to these formations. 

 That no fixed acids are present has not as yet been shown with 

 certainty (although Omeliansky points to their absence) while 

 no volatile acids have been found in an Azotobacter culture by 

 Krzmieniewsky. Repeated trials made in this laboratory in 

 the hope of finding whether the unaccounted for sugar could be 



