STUDIES ON AZOTOBACTER CHROOCOCCUM BEIJ. 



351 



Before entering into a discussion of the above data other 

 experiments will be related. 



Experiment 81. Twenty-five cubic centimeters of the solu- 

 tion mentioned in the previous experiment were placed into 1500 

 cc. Erlenmeyer flasks together with 0.25 gram of CaCOs and 

 sterilized, inoculated with equal and very small quantities of 



TABLE 16 

 Sugar consumed per unit of dry matter in cultures of Azotobacter — experiment 81 



Pronounced opalescence of solution too little to be weighed. 



* S:c.t, where <S sugar consumed, c cellular dry matter and t time in days. 



t This value is in reality calculated differently from the others. But since 

 on the second day of incubation no appreciable amount of cell substance was 

 formed (experiment 81, no. 8) we should be justified in calculating this value just 

 as the others, i.e., on the base of a two days' growth; thus a "ferment power" 

 = 5.45. 



Azotobacter chroococcum and incubated at 30°C. for varying 

 periods of time. The same analytical technique was used in the 

 analysis of these as in the cultures of the previous experiment. 

 Comparing now the data presented in tables 14, 15 and 16 we 

 see that the culture incubated thirty days gave a S:c ratio of 



