GROWTH OF CERTAIN ANAEROBES 



427 



In figure 2 the results are graphically represented. The re- 

 sults from the tables 6 and 7 are almost similar. In both cases 

 there is a marked proteolytic activity between pH 4 and pH 8, 

 and the optimum is in both cases near pH 6. When allowed 



TABLE 6 



Clostridium sporogenes on gelatin 



Filtrate from Clostridium sporogenes A. Klein-Metchnikoff. 

 6 cc. gelatin, 0.5 cc. filtrate 



Temperature, 37' 



TABLE 7 



Clostridium histolyticum on gelatin 



Filtrate from Clostridium histolyticum Weinberg and Seguin. 

 6 cc. gelatin, 0.5 cc. filtrate 



Temperature, 37° 



to digest for a long period of time all tubes except the most acid 

 ones are liquefied. 



We seem justified in stating that in the filtrates from these 

 two microorganisms enzymes resembling trypsin are present. 



