466 HILDA HEMPL HELLER 



Obscure fevers, measles, scarlet fever. Blood cultures, look for various 



invaders. 

 Rhinitis, Vincent's angina; mucous surfaces. 



Make smears of affected tissue, make meat or brain cultures and 

 make at the same time numerous shakes in deep liver-agar. Ex- 

 amine shakes twelve hours after incubation if possible, and 

 examine meat cultures twenty to twenty-four hours after incu- 

 bation. Blood cultures in broth, meat or agar should always be 

 made, if possible, ante mortem and post mortem. 



I wish to express my thanks to Dr. Karl F. Meyer for his active 

 interest and cooperation in this work. 



SUMMARY 



As an aid to the isolation of anaerobes the following notes may 

 be observed : 



1. Success in the isolation of anaerobes depends more on the 

 critical sense of the worker than on the method he employs. 



2. Microscopic observation should be made of incoming ma- 

 terial and of cultures after twenty-four and forty-eight hours' 

 incubation, and the development of a critical eye for the mor- 

 phology and staining reactions of anaerobes is imperative. 



3. Heating of material should be executed according to the logi- 

 cal requirements for that material. Heating at 70° in pipettes is 

 to be recommended for routine work. 



4. A routine medium should be employed which will favor as 

 many diverse forms as possible. Chopped beef heart, preferably 

 containing a little peptic digest broth, the reaction at about pH 7.2, 

 presents numerous advantages as a routine medium, for most 

 of the anaerobes studied in a pathological laboratory. If 

 freshly boiled it is usually quite unnecessary to incubate it 

 anaerobically. 



5. Selective media may be employed for special purposes, and 

 they offer many possibilities. 



6. Isolation by means of guinea-pig inoculation, securing the 

 organism from. the heart's blood or from the affected tissues re- 

 mote from the site of inoculation, is preferable for invading patho- 



