ISOLATION OF ANAEROBES 467 



gens, but may not be depended upon to give a true picture of the 

 pathogenic flora of the material injected. 



7. The making of dilution shakes in deep agar (method of Li- 

 borius and of Veillon) is to be preferred to other colony methods; 

 care must be taken to isolate for a type a colony from an appar- 

 ently pure culture. 



8. A medium for dilution shakes should afford an opportunity 

 for growth to just as many species as possible. Such a medium is 

 pepton-liver agar, as described in the text. 



9. When once pure, a culture should be carefully kept pure. 

 Re-incubation, prolonged incubation in closed jars, storing in 

 closed cans or in dusty places, are to be avoided. Autoclaved 

 media only should be employed for the preservation of type 

 cultures; one cannot be too careful as to routine technique. 



REFERENCES 



Achalme-Passini See Choukevitch (1911). 



Ankekschmitt, p. 1905 Untersuchungen iiber die Bakterien im Verdauungs- 

 kanal des Rindes. Centralbl. f. Bakteriol., O., 39, 359, 574, 687; Ibid., 

 40, 100. 



Barber, M. A. 1914 The pipette method in the isolation of single microor- 

 ganisms and in the inoculation of substances into living cells. Philip- 

 pine Jour, of Science, 19, 307. 



Barber, M. A, 1920 The use of the single cell method in obtaining pure cultures 

 of anaerobes. Jour. Exper. Med., 32, 295. 



Bergey, D. H. 1919 Thermophilic bacteria. Jour. Bacteriol., 4, 301. 



Besson, a. 1913 Practical bacteriology, microbiology and serumtherapy. 

 London. 



Bredemann, G. 1909 Bacillus amylobacter A. M. et Bredemann, in morpho- 

 logischer, physiologischer und systematischer Beziehung. Centralbl. 

 f. Bakt. II. Abt., 23, 385. 



Burke, G. S. 1919 Notes on Bacillus botulinus. Jour. Bact., 4, 555. 



BuRRi, R. See Besson 1913. 



BuRRi, R. 1902 Zur Isolierung der Anaeroben. Centralbl. f. Bakteriol. Abt. 

 II., 8, 533. 



Choukevitch, J. 1911 fitude de la flore bactcrienne du gros intestin du cheval. 

 Ann. de I'Inst. Past., 25, 247. 



Churchman 1912 The selective bactericidal action of gentian violet. Jour. 

 Exper. Med., 16, 221. 



Committee on Anaerobic Infections: Medical Research Committee, Re- 

 ports of the Committee upon Anaerobic Bacteria and Infections. 

 Report on the anaerobic infections of wounds and the bacteriological 

 and serological problems arising therefrom. 1919. Special Report 

 Series, No. 39, London. 



