502 



TH. THjdTTA AND ODD FALSEX SUNDT 



This microorganism was e\'idently seen by Kruse in 1907 

 and called by him tj-pe E (, Kruse, 1907) ; but it was first regarded 

 as a definite tjiDe by Sonne in 1914 (Sonne. 1915) and classed 

 by him in group III. Since then it has been described in France 

 by d'Herelle (1916), in Norway by Thjdtta U919) and in Sweden 

 by OhneU UQIS). 



EXPERIMENTAL 



As a control on our technique, and as a confirmation of the 

 results obtained by Olitsky and Kligler a strain of Shiga bacilli, 

 the first of this form of dysentery- bacilli to be isolated in Norway 

 (by the authors) was examined. 



The Shiga strain was grown in plain broth for eight days, then 

 filtered, and the filtrate (Berkefeld) injected Lntravenously in the foUow- 

 ing rabbits with these results : 



Experiment I. Exotoxin 



Thus it is proved that the filtrate acts as a neurotoxin, pro- 

 ducing distinct paralysis; that a period of incubation precedes 

 the development of these s^Tnptoms; and that the effect is de- 

 pendent upon the amount of the filtrate employed. In none of 

 these rabbits were intestinal lesions found. 



The Shiga strain was grown on agar surface for twenty-four hours, 

 washed off in saline solution, heated for 1 hour at 60°C., and finally 



