TOXINS OF BACT. DYSENTERIAE 



503 



warmed at 37°C. for forty-eight hours. The suspensions were then 

 filtered and injected into rabbits with the following results: 



Endotoxin 



From these experiments it is to be noted that the prominent 

 symptoms w^ere intestinal in origin. At autopsy a marked 

 hemorrhagic colitis was found, similar to that observed in 

 dysentery in man in the stage prior to the development of 

 necrosis. None of these rabbits showed nervous symptoms. 



Thus the results were in accordance wdth the findings of 

 Olitsky and Kligler, and we concluded that the conditions for 

 obtaining a good yield of exotoxin and endotoxin were met by 

 our technique. 



We then proceeded w4th the study of the Bad. dysenteriae, 

 group III. The reaction of the medium during the growth 

 varied as follows: 



TABLE 1 



Medium before inoculation pH 7.6. 



We thus find an initial acid production that is followed by 

 a period of alkalinity. In the end the reaction is more alkaline 

 than at the beginning of the experiment. This confirms the 

 observations of Olitsky and Kligler who also found that the 

 toxin production did not begin until the alkaline period had 



