504 TH. THJ0TTA AND ODD FALSEN SUNDT 



set in. Following the technique of Olitsky and Kligler we collect- 

 ed our toxin after seven days' growth since too prolonged growth 

 tends to yield mixed exotoxin and endotoxin. The broth was 

 now filtered through a Berkefeld filter, the filtrate tested for 

 sterility and the toxin thus prepared was injected into rabbits and 

 white mice. 



Experiment II. Injections into rabbits 



The results w^ere as follows: 



Four hours after the injection the animals became ill. All lay quiet 

 without trying to move. Rabbits 1 and 2 also had quick labored 

 respiration and appeared to be moribund. After this immediate and 

 probably non-specific effect the animals returned to normal as to 

 appearance. If they were disturbed, however, they did not jump about 

 as normal rabbits do, but dragged themselves along with a distinct 

 weakness of the hind legs. 



The weakness of the hind legs was most distinct in the second and 

 third day of the experiment; thereafter it gradually disappeared. 



The loss of weight was as much as 500 grams (rabbit 1). 



A control rabbit injected with 3 cc. of sterile broth did not show any 

 symptoms. 



Additional experiments were made but as the results were 

 similar the protocols are not given. The injection of filtered 

 broth cultures of dysentery bacilli of group III thus causes an 

 effect in the rabbits characterized mainly by distinct paresis 

 of the extremities. No diarrhea was observed and no deaths 

 occurred. 



Endotoxins of Bad. dysenteriae, group III. The endotoxin 

 was prepared as follows: 



Large flasks of agar (diameter 18 by 10.5 cm.) were incubated after 

 inoculation with the strains used in the experiments on exotoxin. A 



