TOXINS OF BACT. DYSENTERIAE 



505 



twenty-four hours' growth of the baciUi was emulsified in normal saline, 

 and the emulsion placed in the incubator for autolysis for two days. 

 It was then filtered through Berkefeld candles, tested for sterility and 

 injected into rabbits. 



Experiment III. Injections into rabbits 



FOURTH DAY 



Well, weight 550 grams 



Well, weight 850 grams 

 Well, weight 2650 grams 

 Well, weight 2100 grams 



Thus, the rabbits after being injected with the endotoxins 

 of the dysentery bacillus of group III showed intestinal disturb- 

 ances as indicated by the diarrhea. One animal died following 

 a large dose (3 cc.) after having shown a profuse blood-stained 

 discharge from the intestines. One animal did not show any 

 sign of diarrhea. In no case was there paresis, and recovery 

 followed promptly. 



Experiments on mice 



Kraus and Doerr studied the effects of the toxins of the Shiga 

 bacillus on hens and pigeons and found they were refractory. 

 Doerr likewise found that guinea-pigs were not affected. We 

 undertook the study of the effects of both the exo- and endo- 

 toxin on mice with the following results: 



* Sterile broth. 



