506 



TH. THJ0TTA AND ODD FALSEN SUNDT 



Four hours after the injection mice 1 to 5 had a profuse bloody 

 and slimy discharge from the anus, the stool hanging in drops from the 

 anal opening. All the mice were sick, huddhng together and showing 

 raised hair. Thirty-six hour after the injection the animals were all 

 right. Mouse 6 did not show any S3m[iptoms at all. 



All the sick mice had a diarrhea of blood-stained mucus after the 

 injection, developing very soon (one and a half hours after injection). 

 At autopsy there was enterocolitis and the lumina of the intestine 

 contained blood-stained mucus. 



Considering the rapid development of the symptoms in mice 

 and the uniformity of the latter in both experiments it is probable 

 that the symptoms were non-specific, at least where the exotoxin 

 is concerned. As to the endotoxin, this certainly made the 

 mice very sick and even killed them, the toxicity of the filtered 

 broth thus being certain. However whether this toxic action 

 was only due to the non-specific bacterial protein toxicity or 

 indicated the specific action of a dysentery toxin we cannot 

 state with certainty. 



Antitoxins 



Our next step was to study the production of antitoxins in the 

 blood of immunized animals and to determine whether these 

 antitoxins were capable of neutralizing the action of both the 

 exotoxin and the endotoxin. For this purpose strong full-grown 

 rabbits were used. The first rabbit receiving exotoxin died 

 from a fulminating intoxication after three injections. The next 

 animal bore the injections well and was given 10 doses of 1 cc. 



