574 J. E. RUSH AND G. A. PALMER 



It was naturally noted (as previously reported) that a greater 

 number of inoculations show liquefaction at 20°C. as time pro- 

 gresses (comparing results at 20°C. for ten days with those at 

 the same temperature for fourteen days). It should also be 

 noted that certain cases show a definite increase in percentage 

 liquefaction from the ten to the fourteen day period. The 

 number of tubes which show liquefaction at fourteen days and 

 none at ten days is however less than one-half of one per cent 

 of the total cultures examined. 



As a general thing more cultures show liquefaction, or there 

 is an increased amount of liquefaction, at 20°C. for fourteen 

 days than by the method of exposure to 37°C. for four days and 

 to 20°C. for one day. 



In those inoculations in which a greater liquefaction by the 

 more rapid method was noted, it may be pointed out that in 

 practically every case total liquefaction occurred. Whether 

 or not this has any significance we are unable to say. 



