42 



SELMAN A. WAKSMAN AND JACOB S. JOFFE 



TABLE 4 



Change in reaction (pH values) produced by the growth of Actinomyceles in culture 

 solutions containing different proteins* 



ORGANISM 



Control 



A. violaceus-ruber . . 



A. f radii 



A. scabies 



A. pheochromogenus 



206 



A. chromogenus 205 



A. aureus 



161 



168 



A. albus 



A. albosporeus . . . . 



A . alboflavus 



A . exfoliatus 



A . griseus 



A. rutgersensis . . . 



A. reticuli 



A. reticulus-ruber . . 



A. lavendulae 



A . halstedii 



A . diastaticus 



A. poolensis 



A . madurae 



A . hominis 



96 



104 



128 



145 



154 



216 



15 PER CENT 



GELATIN 



15 PER CENT 



GELATIN 



AND 1 PER 



CENT STARCH 



6.2 

 8.0 

 5.8 

 8.2 

 8.4 

 7.7 

 7.4 

 7.6 

 7.6 

 8.0 

 6.6 

 7.1 

 6.4 

 5.8 

 7.6 

 8.0 

 7.6 

 6.6 

 5.6 

 5.6 

 6.4 

 7.6 

 6.4 

 7.7 

 8.0 

 6.4 

 7.6 

 6.2 

 5.8 

 6.0 



GLUCOSE 

 BROTH 



7.9 

 6.6 

 6.8 

 7.8 

 8.0 

 8.2 

 7.6 

 5.6 

 8.2 

 8.1 

 7.1 

 7.5 

 7.2 

 7.2 

 8.4 

 7.8 

 7.3 

 5.2 



7.6 

 7.5 

 8.0 

 8.0 

 5.2 

 8.3 

 8.6 

 8.6 

 6.4 

 7.4 

 5.8 







+ + + 

 + 



+ + 



+ + 



+ + 



+ + + 







+ + + 



+ + 



+ + + + 



0- + 



+++ 



++ 



++++ 



++ 





 



++++ 



++ 



+ 



++++ 



++ 

 +++ 

 +++ 



++ 



++ 





 



++++ 



* The gelatin cultures were incubated for thirty days at 18°, glucose broth 

 at 25° and milk at 37° for fifteen to twenty days. 



obtained) than in the corresponding culture on gelatin where the 

 starch was absent. But no hasty conclusion should be drawn 

 that these organisms produce acid out of the starch; there was 

 probably no acid produced, but the presence of starch resulted 

 in a change in the utilization of the gelatin, which produced a 



