STERILIZATION OF OILS 



59 



of effective sterilization of the oil. The amount of enzyme pres- 

 ent in the raw oil must be small, since the amount of acid present 



TABLE 4 

 Lipase suspension in cotton seed oil 



CONDITION 



Raw oil. 



Dried and filtered oil. 



Dried, filtered suspension. 



EXPOSURE 



minutes 









 





 



3.5 



5.0 



10.0 



INCUBATION 

 PERIOD 



hours 









 18 





 18 

 18 

 18 

 18 



N/100 NaOH 



REQUIRED 



0.2 



0.2 

 1.4 



4.0 



50.0 



13.0 



9.1 



5.4 



TABLE 5 

 Lipase suspension in cotton seed oil 



after eighteen hours incubation with distilled water serves to 

 increase the amount of N/100 NaOH required from 0.2 cc. to 

 1.0-1.4 cc. only. 



