MODIFICATION OF LOEFFLER's FLAGELLA STAIN 187 



mordant which was Bunge's modification. By using one drop 

 of this solution with about eight drops of the ferric tannate solu- 

 tion, applied together on the cover-glass, all necessity of heat- 

 ing the mordant or the stain is obviated. 



The stain recommended is a special methylene blue made by 

 adding an alcoholic solution of anilin-oil (1:4) to Loeffler's 

 methylene blue in the ratio of 1 : 10. 



The chief advantages of the process are (1) its simplicity, (2) 

 the use of solutions that keep well, (3) the use of all solutions at 

 room temperatures, and (4) the high percentage of successful 

 attempts, even in the hands of inexperienced students. 



REFERENCES 



Bunge, R. 1894 Ueber Geisselfarbung von Bakterien. Fortschr. d. medizin, 



Bd. 12, No. 12, 462-464. 

 Chester, D. D. 1901 A Manual of Determinative Bacteriology. 6-7. 

 Ellis, D. 1909 Outlines of Bacteriology. 18-20. 



Frost, W. D. 1903 A Laboratory Guide in Elementary Bacteriology. 48-50. 

 Giltner, Ward 1916 Laboratory Manual in Elementary Microbiology. 



93-95. 

 Jordan, E. O. 1914 A Text-book of General Bacteriology. 49-50. 

 Loeffler, F. 1889 Eine neue Methode zum Farben der Mikroorganismen, 



besondern ihrer Wimperhaare und Geisseln. Centralb. f. Bakt. 6, 209-224. 

 Loeffler, F. 1890 Weitere Untersuchungen liber die Beizung und Farbung 



der Geisseln bei den Bakterien. Centralb. f. Bakt., 7, 625-639. 

 Loewit, N. 1896 Zur Morphologie der Bakterien. Centralb. f. Bakt., 19, 



673-686. 

 Mallort, F. B., and Wright, J. H. 1901 Pathological Technique. 102-106. 

 Muir, R., and Ritchie, J. 1913 Manual of Bacteriology. 111-113. 

 Park, W. H., and Williams, Anna W. 1914 Pathogenic Microorgnisms. 



80-81. 

 Smith, Erwin F. 1905 Bacteria in Relation to Plant Diseases. 20-21, 190- 



193, 219-220. 

 Sternberg, G. M. 1896 A Text-book of Bacteriology. 32-33. 

 Van Ermengem, E. 1893 Nouvelle methode de coloration des cils des bac- 



te>ies. Trav. du. Lab. d. Hygiene et de Bact. de I' Univ. de Gand. T. I., 



1, 3. 

 Ward, H. M., and Blackman, V. H. 1910 Bacteriology. Encyclopaedia 



Britannica 3, 159-160. 



