302 G. E. BURGET 



the number of different organisms is few and they are unrelated 

 making it impossible to classify them into a definite group or 

 groups. Extracts with acidity below normal have a much higher 

 bacterial content and a greater variety of organisms. 



Not being able at the time this work was being carried on to 

 spend further time in the study, a somewhat incomplete descrip- 

 tion of the following organisms is made necessary : 



No. 7. A yeast. Colony on glucose agar, round with rough edges 

 made of projecting filaments; white to gray with raised center giving 

 nail head appearance (see photograph). 



Milk. No acid. 



Lactose broth: flocculent precipitate after two weeks, no fermenta- 

 tion, no gas, no acid. 



Glucose agar stab: no gas. 



Glucose litmus agar: Acid formed. 



Gelatin: Not liquefied. 



No. 4- A yeast. Colony heavy gray on glucose litmus agar, small, 

 acid forming. 



Glucose agar slant: Heavy gray waxy growth. 



Milk: Acid formed; proteolysis. 



Gelatin: Liquifaction. 



Lactose broth: Filaments formed; no gas. 



Resembles bacterium, has granules and vacuoles; Gram positive. 



No. 5. Bacillus. 



Colony: Thin, gray and rather large. A short bacillus forming 

 chains. 



Glucose litmus agar: No acid. 



Glucose stab: No gas. , 



Litmus milk: Decolorized, no coagulation. 



Lactose broth : No gas, no acid, gray precipitate. 



Gelatin: Not liquified, (growth at 20 degrees C). 



Non motile, spore forming, Gram positive. 



No. 8. Coccus form. 



Heavy colony slightly yellow on Russell. 



Glucose stab: No gas, no acid. 



Lactose broth: Cloudy but not much precipitate, no acid, no gas. 



Milk: No acid. 



Large cocci with tendency to pairs. 



Gram positive. 



