PLATE 1 



Fig. 1. Azo. 1. Smear from a twenty-four hour culture on Ashby's agar at 

 25°C, inoculated from a seven-days old culture in Ashby's solution in which 

 rapid growth was occurring and many cells were disintegrating. Smear shows 

 very young cells presumably developed from the regenerative granules dispersed 

 by disintegrated mother cells. Central corpuscles, possibly nuclear bodies, are 

 noticeable in most of the young cells and fission in all stages is apparent; cells 

 were actively motile. Stained with saturated alcoholic gentian violet. X 1000. 



Fig. 2. Azo. 1. Cells showing flagella from same culture as figure 1, stained 

 with Moore's modification of Loeffler's flagella stain. X 1000. 



Fig. 3. Azo. 4. Resting cells or arthrospores germinating. Preparation in 

 Lugol's solution from a twenty-four hour culture on Ashby's agar at 25°C, inocu- 

 lated from a one-month old culture in which many resting cells were present. The 

 dark, granular irregular spherical bodies are the resting cells and the light-colored 

 rods are from the germinated resting cells. X 1000. 



Fig. 4. Azo. 3. Smear from a four-day old culture on Ashby's agar at 25°C, 

 showing fission first in one direction, then at right angles, giving tetrad forms 

 common with this variety. Stained Neisser's blue. X 1000. 



Fig. 5. Azo. 1. Smear from culture on Ashby's agar, six days old at 25°C, show- 

 ing capsule formation and development of cells in many stages from granules dis- 

 persed from disintegrated mother cells. Granular mother cells also in evidence. 

 Stained with saturated alcoholic gentian violet. X 1000. 



Fig. 6. Azo. 2. Smear from culture fourteen days old on Ashby's agar at 25°C. 

 showing resting cells or arthrospores. Stained with saturated alcoholic gentian 

 violet. X 1000. 



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