PLATE 3 



Fig. 1. Azo. 4. Smear from growth five days old on Ashby's agar at 25°C, 

 showing various sized rods positive to the stain and granular spheres mostly nega- 

 tive to the stain, also a cluster of cells probably entering the symplastic stage 

 described by Lohnis and Smith. Stained Heidenhain's iron hematoxylin. X 

 1000. 



Fig. 2. Azo. 4. Smear from culture sixteen days old in Ashby's solution at 

 25°C, showing at the top a portion of a mass of cells entering the "symplastic" 

 stage; at the bottom a portion of a mass of cells in an advanced "symplastic" 

 stage where apparently the cells have fused and the reproductive granules are 

 coming into evidence ; at the right hand side a number of scattered young cells 

 developed from reproductive granules liberated from another mass of "sym- 

 plasm." Stained Neisser's blue. X 1000. 



Fig. 3. Azo. 3. Smear from a streak culture three weeks old on a beef extract 

 agar plate inoculated from an Ashby solution culture seven days old, showing a 

 few tetrad forms of resting cells and to the left a portion of a "symplasm" in an 

 advanced stage; to the right numerous minute reproductive granules escaped 

 from the symplasm. Around each individual strongly stained granule is a zone 

 of plasma negative or only slightly positive to the stain, clearly discernible in the 

 smear but not showing up well in the photograph. Stained Neisser's blue. X 

 1000. 



Fig. 4. Azo. 3. Smear from colony one month old on beef extract agar showing 

 darkly stained clusters of resting cells also clusters of young cells developed from 

 reproductive granules from disintegrated mother cells. Stained Kutscher's 

 gentian violet; X 1000. 



Fig. 5. Azo. 4. Smear from sixteen days old culture in Ashby's solution 25°C, 

 showing two aggregations of cells probably entering "symplastic" stage and in 

 the center a mass of young cells developed from regenerative granules. Stained 

 Neisser's blue. X 1000. 



Fig. 6. Azo. 3. Smear from a colony six days old on Ashby's agar at 25°C, 

 showing granular mother cells disintegrating, dispersal of granules and various 

 stages in development of reproductive granules into young cells multiplying by 

 fission. Stained with saturated alcoholic gentian violet. X 1000. 



338 



