374 



P. J. DONK 



Agar colonies: Growth moderate; temperature 60° to 65°C. form, 

 circular; surface, smooth; elevation, flat; edge, regular; maximum 

 diameter observed 2 mm., white opaque spot in center surrounded by 

 several concentric rings. 



Gelatin: Temperature 20°C, no growth; 60° to 65°C. growth but no 

 liquefaction. (Inoculated tube showed uniform clouding after incuba- 

 tion for three days, solidified when placed in ice-box.) 



Glucose agar stroke and stab (litmus): Growth, acid throughout. 



Lactose agar stroke and stab (litmus): Growth, acid throughout. 



Sucrose agar stroke and stab (litmus): Growth, acid throughout. 



Potato: Growth, none. 



Potato starch agar: Growth copious. 



Corn infusion (litmus) : Strong acid after twenty-four hours. Starch 

 digested. 



Temperature relations: Optimum temperature for growth 50°C. 

 Maximum 76°C. Minimum 45°C. 



Litmus milk: Growth begins after twenty-four hours, acidified, 

 litmus partly reduced. No coagulation after forty-eight hours. After 

 four days litmus completely reduced and casein digested, leaving 

 heavy sediment in bottom of tube. (Control tube showed loss of color 

 only after six days, no coagulation, no digestion.) 



Indol production: Absent. 



Nitrate broth: Not reduced; ammonia, none. 



Glucose broth: Gas production, none. 



Lactose broth: Gas production, none. 



Sucrose broth: Gas production, none. 



The thermal death relations at two temperatures and 

 media containing different numbers of spores are as follows : 



m 



