422 J. RUSSELL ESTY 



I. A male guinea-pig, weighing 571.5 grams was inoculated sub- 

 cutaneously with a 2 cc. glucose broth culture 7 of cow feces. Although 

 there was a loss in weight to 547.5 grams at the end of the first day, 

 no discomfort was observed and the pig was lively and in a healthy 

 condition. In forty-eight hours it had gained slightly, weighing 553 

 grams and its weight remained quite constant for the next few days. 

 This injection was made on January 31, 1917, and proved non-patho- 

 genic. On February 6, the same pig weighing 548 grams was reinocu- 

 lated with a pathogenic strain to see if any immunity had been pro- 

 duced. Two and three-quarters cubic centimeters of number seven 

 culture from human feces was used subcutaneously. A reaction 

 occurred but did not prove fatal. The pig lost weight consistently, 

 dropping to 457 grams on February 19, thirteen days after the inocula- 

 tion. A local infection occurred with swelling of the abdomen and gas 

 in the tissues. A large open abscess was formed along the entire right 

 side of the abdomen. On February 19, the abscess began to heal and 

 the animal gained consistently, recovering completely in twenty days. 

 A control pig was inoculated with number seven human feces and died 

 in fifteen hours. It was evident, therefore, that immunity had been 

 produced to a high degree by the previous injection of a non-pathogenic 

 strain. 



On March, 20, 1917, this same pig now weighing 528 grams and in 

 excellent condition was inoculated again subcutaneously with 2.5 cc. 

 of a fresh culture of a vegetative organism from human feces. Local 

 swelling was produced and loss in weight was consistent to 467 

 grams when the abscess broke and recovery seemed probable. Two 

 days after another abscess appeared extending to the neck which 

 further weakened the resistance of the pig and resulted in another loss 

 of weight from 467 to 350 grams. Although the abscess had prac- 

 tically healed the pig was found dead on the fourteenth day. The 

 cause of the death was not primarily due to the infection of Clostridium 

 Welchii, but after the resistance had been lowered to such an extent 

 the pig was unable to survive. A smaller dose should have been 

 injected, although some immunity was no doubt produced. The con- 

 trol pig died thirty hours after the injection of a 2 cc. culture subcu- 

 taneously. 



II. A male guinea-pig weighing 499 grams, was inoculated subcu- 

 taneously on February 6, with 2 cc. of culture 5 from cow feces grown 

 in sterile milk. A normal infection occurred resulting in gas in the 

 tissues and a pronounced swelling. Loss in weight continued for two 



