438 o. ishii 



others long (just the same results were obtained with or without 

 an indicator, either litmus or china blue). 



In the glucose-peptone water cultures in most cases, Bact. 

 typhi died in three to five days, Bact. paratyphi A in three to 

 five days, Bad. paratyphi B in three to six days, Bad. enteritidis 

 in five to seven days, Bad. suipestifer in seven days, Bad. coli 

 in six to seven days, Proteus in seven days and Bad. dysenteriae 

 in three to seven days; but all strains of Bad. pyocyaneus still sur- 

 vived after thirty days' growth. The above results with Ps. 

 pyocyanea were obtained with strains received from Dr. C.-E A. 

 Winslow, and from Dr. Oscar Teague, Medical School of Columbia 

 University of New York; a few strains, which we have isolated 

 from feces, however, died within ten days in glucose broth, while 

 others survived more than thirt}' days. It would seem that 

 there are a number of different types of Ps. pyocyanea with dif- 

 ferent biological properties. 



In mannitol peptone water cultures, in most cases, Bad. typhi 

 died in four to six days, Bad. paratyphi A in four to five days, 

 Bact. paratyphi B in five to eight days, Bad. enteritidis in about 

 eight days, Bact. suipestifer in about eight days, Bact. coli in five 

 to eight days, Bact. dysenteriae in five to ten days (with the ex- 

 ception of one which survived for over thirty days) ; Proteus and 

 Bact. pyocyaneus were still alive after thirty days. One special 

 strain of Bact. dysenteriae Shiga # T (obtained direct from Dr. 

 Shiga, Japan), was still alive after thirty days, though another 

 strain of Shiga no. 1, was dead in ten days. This is very peculiar, 

 since culturally both were otherwise the same and there was no 

 acid fermentation in either case. 



• In the lactose peptone water cultures of all the strains of Bact. 

 typhi, Bact. paratyphi A, Bad. paratyphi B, Bact. enteritidis, 

 Bact. suipestifer, Proteus, Bact. pyocyaneus and of all of the Bact. 

 dysenteriae group, none had died within one month, but all strains 

 of the Bact. coli group died within five to eighteen days. 



In the sucrose peptone water cultures of all the strains used 

 of Bact. typhi, Bact. paratyphi A and B, Bact. enteritidis, Bact. 

 suipestifer, Proteus, Bact. pyocyaneus and of all of the Bact. dysen- 

 teriae group, none died within a month. Of the seven strains of 



