472 



C. P. FITCH AND W. A. BILLINGS 



The strains of Bad. abortivo-equinus were obtained as follows: 

 GI, Gil, and GUI, were sent us by Professor Good of Kentucky, 

 M.7 and M.8 from the Bureau of Animal Industry, Washington, 

 D. C., H.3 and H.13 from the Laboratory of the Pennsylvania 

 Live Stock Sanitary Board, Philadelphia. Each strain was in- 

 oculated in a series of the heated and unheated media. A tube 

 was left uninoculated as a check for each strain which was incu- 

 bated with the others. In as much as the observations of Good 

 and Meyer were made on the basis of the reaction of the culture, 



Table showing the results of gas production by Bad. abortivo-equinus in heated and 



unheated carbohydraU media. Also a comparison with the results 



in heated media of other investigators 



* None in one trial; very slight in another. 



using phenolphthalein as an indicator, it was deemed advisable 

 to continue with this system instead of employing the more 

 accurate pH standard. The tables at the end of the article give 

 the results of these observations, continued in nearly all cases 

 over a period of thirty days. In some instances it was impossi- 

 ble to obtain at the time sufficient of the carbohydrate to com- 

 plete the series. The figures give the amount of & NaOH or 

 to HC1 (if minus sign precedes) necessary to neutralize five mils 

 of the culture. 



