504 



TH. THJCfTTA AND ODD KINCK EIDE 



TESTED STRAINS 



Atypical. . 

 Typical. . . 

 Para. B 16 



ABSORPTION WITH 



ATYPICAL IN HO- 

 MOLOGOUS SERUM 



100 

 200 

 800 



ABSORPTION WITH 



NORMAL IN HO- 

 MOLOGOUS SERUM 



200 

 200 

 400 



ABSORPTION WITH 



PARA. B IN HO- 

 MOLOGOUS SERUM 



200 



800 



1600 



These tests were carried out in cultures from well isolated 

 colonies, but not with one cell cultures. As it, however, was 

 thought advisable to exclude the possibility of our working with 

 cultures containing both atypical and typical bacilli, we prepared 

 one cell cultures after the method of Burri, and made the follow- 

 ing serological tests with cultures, that had been obtained from 

 but one atypical bacillus, and that in growth were just as atypi- 

 cal as the cultures had been before. 



a. Agglutination in paratyphoidserum : 



b. Absorption test after Castelliano with atypical strain in 

 6 hours (antigen added twice) : 



Agglutination titer after six hours in the incubator 



Typical strain 400 



Atypical strain 200 



c. Complement absorption test : 



Antigen. One fiftieth of a twenty-four hours old culture (agar slope), 

 emulsified in saline and killed by heat. 



Serum. Paratyphoidserum produced with a standard paratyphoid B 

 strain. Dose: 0.05-0.0001 cc. 



Complement. Fresh guinea-pig serum, dose: 0.06 cc. 



Time of absorption. Six hours at 8°. 



Blood corpuscles of sheep, washed and emulsified as in the test of 

 Wassermann. 



Hemolytic amboceptor. As in test of Wassermann. 



