BACTERIAL INHIBITION 



529 



glass-stoppered bottles were used for inoculation, and equal 

 samples were kept for controls. 



The work of Coplans, Rahn, Penfold, Chesney, Salter, and 

 others on the latent period of growth in freshly inoculated bouil- 

 lon has demonstrated that the most favorable conditions for 

 transferring a culture are during its period of logarithmic increase 

 and between media of the same composition at the same tem- 

 perature. Therefore a constant temperature of 37°C. was used 



TABLE 1 

 Growth of bacteria in raw milk at 37°C. 



throughout the experimental work to give a more intense action 

 over a shorter period of time of observation. The milk inocula- 

 tions, 1 cc. portions from five-hour milk cultures at 37°C, were 

 high compared to the germ content of the raw milk, thus mini- 

 mizing the influence of the original udder flora upon the growth 

 in the raw inoculated milk. Numerical determinations were 

 averaged from triplicate plates of 1 per cent lactose agar, made 

 essentially according to the standard methods of the American 

 Public Health Association. 



