BACILLUS BOTULINUS AND ITS SPORES 



555 



Strains Cal I, Cal II, Chi 595, Mul 435, and AM of NH, are 

 from the same original source and are of low virulence, and the 

 results are practically the same, i.e., growth occurred throughout 

 in all percentages of salt, and their vitality was not apparently 

 impaired by a sojourn for 49, 27 or 31 days respectively in 10 



TABLE 1 



Resistance of B. botulinus 



° Alb 175 negative. 



00 Alb 175 and Alb 175B both negative. 

 000 Alb 175B negative. 

 0000 Strain Mul 471 doubtfully positive at this time. 



* Strains Cal II, Cal III, Cal IV, and Cal VI only, examined. 

 ** Strain Cal I only, examined. 

 *** Strain Cal III, only, negative. 



per cent salt broth. These results are not confined to strains 

 of low virulence, however. Similar results were obtained with 

 strains of high virulence such as Cal IV, NBS, G06, Mul 433 

 and Alb 175B, which survived 10 per cent salt for 49, 32, 27, and 

 27 days respectively. Cal III, Cal VI, and Mul 471, virulent 

 strains, were the only strains virulent or non-virulent showing 



