W. Taylor and A. D. Husband 



113 



Albumin and Globulin. These were precipitated by tannic acid from 

 the filtrate obtained in the estimation of casein, after which they were 

 estimated together as described above for casein. 



Non-Protein Nitrogen. This was estimated by the difference between 

 the percentage of total protein and the sum of the percentages of casein, 

 albumin and globulin. 



Fat. By the Soxhlet method. 



Lactose. Fehhng's method and Benedict's method were both made 

 use of; but in the individual experiments one method was adhered to 

 throughout. 



A.sh. By the ordinary method of ignition. 



EXPERIMENTAL. 



A. The Effect of Variations in Volume on the Percentage Com- 

 position OP THE Milk, with an Animal on a Diet of Constant 

 Composition. 



Experiment I. (Analytical Data, Table I.) 

 In this experiment a series of milk analyses was carried out from 

 day to day in the case of a goat fed on a diet of hay and a mixture of 

 meals made from locust beans, earth nuts and oats, this mixture being 

 sweetened with sugar. The animal was allowed to eat to its appetite, 

 but the nature of the diet was constant throughout. Owing to the con- 

 finement, the monotonous diet and the advanced stage of lactation, the 

 volume of the milk fell steadily throughout the experiment with, as a 

 rule, but slight variations from day to day. 



Table I. 



