G. Williams 189 



The remaining solution was evaporated down to dryness, and a 

 small quantity of alcohol added to the sticky residue. A saturated 

 solution of picric acid in alcohol was then added, but only a small preci- 

 pitate was obtained. It was allowed to stand for twenty-four hours, 

 and the lysine picrate filtered ofi. More picric acid was added and 

 more picrate filtered ofi. This was repeated again. The lysine mother 

 liquor was now acidified with sulphuric acid, the picric acid extracted 

 with ether, the lysine precipitated as phosphotungstate, and the above 

 process for obtaining lysine picrate repeated. 



Total weight of recrystaUised lysine picrate obtained = 3*28 grms. 



Allowing for portions removed for analysis this becomes 3-86 grms. 



= 1-503 grms. lysine. 



This gives a content of lysine in the protein of 4-35 per cent. 

 The picrate exploded in a melting-point tube at 246° C. It was 

 analysed both by the Kjeldahl and Dumas methods. 



0-1177 grm. gave ^^THg equal to 15-75 c.c. N/10 acid 



= 0-02205 grm. N 



= 18-74 per cent. N. 

 Lysine picrate contains 18-67 per cent. N. 

 0-1039 grm. gave 17-35 c.c. Nitrogen at 2V' C. and 758 mm. 



= 0-01935 grm. nitrogen 



= 18-63 per cent, nitrogen. 

 Lysine picrate contains 18-67 per cent, nitrogen. 



Glutaminic and Aspartic Acids, Glycine, Alanine, Valine, the 

 Leucines, Phenylalanine and Proline. 



A weight of the protein equal to 288-08 grms. of the dry ash-free 

 substance was hydrolysed by boiling with three times its weight of 

 concentrated hydrochloric acid. The flask was first warmed on the 

 water bath until solution was complete; it was then heated in an oil 

 bath at a mean temperature of 1 18° C. under a reflux condenser for 

 forty-four hours. At this temperature the liquid boiled fairly briskly. 



Separation of humin substances. 



The cooled solution was filtered through a Buchner funnel covered 

 with linen and the black insoluble matter thoroughly washed. 



Weight of dry humin bodies obtained = 13-65 grms. 



= 4-74 per cent, of the protein. 



13—2 



