198 Hiidrohjsis of the Soluble Protein of Swede Turnips 



Fraction 1. The 3-2 grams of acids were dissolved in nearly four parts 

 of hot water and 4 grams of picric acid added. The mixture was 

 cooled to 0° C. and allowed to stand for two hours. The yellow crystals 

 which separated were filtered ofi in a cooled funnel, washed once with 

 a few c.c. of cold water, and three times with cold 95 % alcohol. The 

 crystals thus obtained weighed 0-9 grm. and softened at 200° C, decom- 

 posing at 202° C. The filtrate was now treated with 45 c.c. of N 

 sulphuric acid, and the picric acid extracted with ether. The sulphuric 

 acid was then removed quantitatively by means of 130-4 c.c. of cold 

 saturated baryta water. The barium sulphate was filtered ofi and the 

 filtrate and washings evaporated down to dryness. The residue was 

 recrystalhsed from water and the acids left in the mother hquor weighing 

 0-87 grm. treated for a further yield of glycine picrate as described 

 above. 0-62 grm. of glycine picrate was obtained which decomposed 

 at 202° C. Attempts were made to obtain glycine picrate from 0-5 grm. 

 portions of crops 2 and 3, but with no success. The picric acid was 

 removed from these test portions and the acids put back. 



Ether and chloroform distillates treated for glycine. 



The ether distillate from the esters (second distillation) was shaken 

 up with dilute hydrochloric acid, and the acid extract evaporated to 

 dryness. Practically no residue was obtained. The chloroform dis- 

 tillates however, when treated in a similar manner, gave a residue 

 of 1-6 grms. of acid hydrochlorides. This was treated with silver 

 sulphate solution to remove the chlorine, the excess of silver removed 

 by hydrogen sulphide, and the sulphuric acid by baryta. The weight of 

 dry amino-acids thus obtained was 1-02 grms. melting at 136° C— 140° C. 

 and containing 14-54 % N. It evidently contained leucine or vahne 

 along with alanine and glycine. It was recrystalhsed from water and 

 the more soluble part remaining in the mother hquor treated for glycine 

 picrate. 0-280 grm. of glycine picrate was obtained equal to 0-31 grm. 

 allowing for the portion removed for analysis. 



A further small quantity (0-1 grm.) of glycine picrate was prepared 

 from the distillate obtained during the saponification of Fraction 1 

 (see page 194). 



The total weight of glycine picrate obtained was 1-93 grms. equal 

 to 0-76 grm. glycine. This corresponds to a percentage of 0-27 of glycine 

 in the protein. 



