210 Hydrolysis of the Soluble Protein of Sivede Turmps 



A Dumas nitrogen determination was also made. 



0-1409 grm. gave 12-0 c.c. N at 14-5° C. and 769 mm. 

 = 11-4: c.c. at N.T.P. 

 = 10-18 per cent. N. 



Analyses of aspartic and glutaminic acids and of the mixture : 



Aspartic acid Mixture Glutaminic acid 



36-09 per cent. C 37-44 per cent. C 40-81 per cent. C 



5-27 per cent. H2 5-67 per cent. H2 6-12 per cent. H2 



10-52 per cent. N2 10-18 per cent. N2 9-52 per cent. N2 



Calculating the proportions of the acids present in the mixture 

 from the carbon content 



37-44 - 36-09 .r^r.^^^ .r.r.^ 28-60 % glutaminic acid, 

 40-81 - 36-09 ^ " 4-72 ^ 71-40 % aspartic acid. 



Calculating on the nitrogen content 



10- 18-9-5 2 T ^^ _ :66 1 f^f. _ 66 % aspartic acid, 

 10-52 - 9-52 ^ ^^" ~ 1 ^ ^^^ 34 % glutaminic acid. 

 Taking the mean of the two we get 31-3 per cent, of glutaminic 

 acid and 68-7 per cent, of aspartic acid. The mixture was therefore 

 made up of 1-046 grms. of glutaminic acid and 2-296 grms. of aspartic 



acid. This means percentages of 09. qa ^ 100 -= 3-18 of glutaminic 



9,295 

 acid, and '^ — ^ x 100 = 6-98 of aspartic acid m the protein 



Separation of Glutaminic and Aspartic Acids. 

 Preparation of copper aspartate from the mixture. 



2-727 grms. of the mixture were dissolved in 200 c.c. of water and 

 excess of copper carbonate added. The mixture was boiled for ten 

 minutes and the excess of copper carbonate filtered off and washed with 

 boihng water. The filtrate and washings were evaporated down until 

 crystallisation commenced. A large crop of the characteristic needles 

 of copper aspartate separated out over night. These were mixed with 

 a small amount of small prisms characteristic of copper glutaminate. 

 These were much heavier than the copper aspartate crystals and thus 

 rendered complete separation by elutriation quite easy. 



The copper aspartate crystals were air dried for a week, and weighed 



