G. Williams 211 



2-4765 grms. equal to 1-48 grms. of aspartic acid. 0-5072 gram of the 

 air-dried copper aspartate gave 0-1460 grm. CuO 



= 0-1165 grm. Cu 

 = 22-98 per cent. Cu. 

 Calculated for C4H5NO4CU . 4| H3O = 23-07 per cent. Cu. 



The heavier crystals separated by elutriation were dried in the 

 oven and weighed 0-6728 grm. These were ground up finely and about 

 100 c.c. of water added. Upon warming, the crystals dissolved partly ; 

 the copper was then removed by means of hydrogen sulphide. The 

 filtrate was evaporated to drjmess and the residue of glutaminic acid 

 weighed 0-4066 grm. 



0-1488 grm. gave 12-1 c.c. N at 15° C. and 768 mm. 



- 0-01439 grm. N 



= 9-66 per cent. N. 

 Calculated for C5H9NO4 = 9-53 per cent. N. 



Isolation of Glutaminic Acid as Hydrochloride from 

 THE Filtrate from the Copper Salts. 



No attempt was made to obtain a second crop of crystals from 

 the filtrate from the copper salts. The copper was removed from the 

 mother liquor by means of hydrogen sulphide and the Uquid evaporated 

 to dryness. The residue weighed 0-841 grm. This was a mixture of 

 glutaminic and aspartic acids left behind in solution as their copper 

 salts. The residue was dissolved in 1-5 c.c. of strong hydrochloric 

 acid, boiled for a minute and then saturated with dry hydrochloric 

 acid gas at 0° C. A heavy crop of glutaminic acid hydrochloride 

 separated out. The Uquid was allowed to stand over night in ice. An 

 equal volume of ice-cold absolute alcohol was then, added and the 

 hydrochloride filtered off through ordinary filter-paper. It was washed 

 with an ice-cold mixture of equal parts of saturated hydrochloric acid 

 and absolute alcohol. The hydrochloride was dried in the oven and 

 weighed 0-5544 grm. equal to 0-444 grm. glutaminic acid. 



0-1591 grm. gave 10-2 c.c. N at 15-5° C. and 760 mm. 



= 0-01199 grm. N 



= 7-54 per cent. N. 

 Calculated for C5H9NO4HCI - 7-62 per cent. N. 

 2-727 grms. of mixture had been taken. Calculating on the basis 

 of 31-3 per cent, of glutaminic acid previously determined, this should 



