220 The Celery-Rot Bacillus 



Broccoli. The youngest of the four leaves inoculated collapsed on 

 the third day; the rot failed to make any decided progress on the 

 older leaves. 



Cauliflower. The three youngest leaves collapsed within three days 

 while two older ones proved resistant. 



Rape. Inoculation led to a slight discoloration and splitting of 

 the tissues at the punctures but no soft rot set in. 



Cabbage. Two of four leaves inoculated collapsed in the course 

 of a few days, the other two only showed a discoloration of the tissues 

 round the wounds. 



ParsnijJ. In one experiment this plant gave negative results. On 

 repeating the experiment and inoculating two very young growing 

 leaves of each of two plants, all four leaves collapsed within 

 24 hours. 



Potato. Tubers were planted in pots and when the shoots were 

 a few inches high inoculations were made directly on the haulm itself 

 at about two inches above the ground level. Of six shoots so treated 

 one collapsed on the fifth day after inoculation and a portion (4 cm. 

 long) of the haulm was destroyed by the typical soft rot (Fig. 2). In the 

 others the wounds increased a little in size but the shoots remained 

 upright. Wounded controls showed no change. 



In a second experiment with potato shoots a modification was 

 introduced in that one pot was covered, for two days previous to inocu- 

 lation, with a bell-jar which was replaced immediately after its temporary 

 removal for the inoculations, and since the soil was kept damp an 

 atmosphere saturated with moisture was maintained. The second 

 pot had been uncovered from the time the tubers were planted and 

 remained uncovered after the shoots had been inoculated. Four 

 haulms were inoculated and four were left as controls in each pot, 

 with the following result. 



Pot A (saturated atmosphere). On the third day after inocula- 

 tion two of the shoots collapsed and after two more days a third ; in 

 the fourth shoot the rot failed to make much progress although the 

 wound increased in size and developed into a longitudinal fissure. 

 The control shoots remained healthy and fourteen days after com- 

 mencing the experiment the punctures were represented in each case 

 merely by a small scar. 



Pot B (dry atmosphere). In 24 hours the inoculations had 

 caused a slight discoloration round the wounds, the affected areas 

 being from 1 to 3 mm. long; although these developed later into 



