226 The Celery-Rot Bacillus 



Thymol. The thymol used was in the form of small particles, the 

 largest being about 1 mm. in diameter; when agitated as little as 

 possible most of these remained floating on the surface of the medium, 

 but when shaken they immediately sank. It was found that 0-1 % 

 even without shaking was sufficient to prevent development. 



Toluene. 1 % when shaken up in the bouillon inhibited growth. 

 When unshaken no growth was apparent with 1 % or 2 % so long 

 as a film of toluene remained on the surface, but after the evaporation 

 of the film growth proceeded normally ; with 4 % however no growth 

 occurred even after the evaporation of the film. 



Alcohol^. With 1 % growth, so far as can be observed, is unaffected, 

 while in 2 % it is slightly retarded (when compared with inoculated 

 control tubes), for although there is evidence of growth at the end 

 of 24 hours turbidity is hardly noticeable and there is no sediment, 

 while in 1 % and in the inoculated controls turbidity is more distinct 

 and a little sediment is thrown down. In the case of 5 % no change 

 was observed up to the sixth day after inoculation ; later however the 

 medium became turbid and a distinct sediment was formed. This 

 suggests that when the medium contains 5 % alcohol the organism 

 is not killed but that development is in abeyance until favourable 

 conditions supervene. As the alcohol evaporated at a more rapid 

 rate than the water of the culture medium a point would eventually 

 be reached at which the amount of alcohol present was not inhibitory 

 to the organism. 



Another experiment was then performed the result of which sup- 

 ported this view. Tubes prepared as in the previous experiment 

 contained respectively 4 %, 5 %, 6 % and 7 % alcohol and were 

 inoculated from a pure culture on Jan. 26th, 1915. The tubes were 

 examined daily and all remained clear for five days ; on the sixth day 

 the tube containing 4 % showed the first signs of turbidity. Growth 

 then commenced in the other tubes in succession with a few days' 

 interval between the first appearance of turbidity in any one tube and 

 in the next of the series as here shown : — 



1 As the results obtained with alcohol appear to be of special interest they are given 

 in detail. 



