TSE-(T-3)-7 



JUNE -SEPTEMBER 1968 



Sample 



Table 1. — Source of Arctic specimens. 

 Date 



Program 

 Sample 



Depth 



(m) 



Figure 2. — Positions and track of Fletcher's Ice Island ("T-3") 

 in the Canadian Basin, June-September 1968. 



Samples were collected in 1-m closing nets with 

 mesh apertures of 0.215 mm (1967) and 0.110 mm 

 (1968). A series of vertical samples was obtained in 

 summer 1967 (July and September), and a second 

 series in summer 1968 (June, July, and August), a 

 total of 142 samples. Depth intervals sampled were: 

 10-100 m, 10-m intervals; 100-200 m, 20-m intervals; 

 200-400 m, 50-m intervals; 400-500 m, 100-m inter- 

 vals; 500-.3,000 m, 500-m intervals. Usually more than 

 one sample was obtained from each interval each 

 summer. Some specimens were obtained from 

 samples not in the above routine series; these samples 

 are included in Table 1, but the specimens are not 

 enumerated in Table 2. 



Samples were fixed in 5^7 formaldehyde/seawater 

 solution, buffered with sodium borate. 



Laboratory Methods 



Arctic specimens were preserved in 4% form- 

 aldehyde/3'f sodium acetate solution; most borrowed 

 specimens were preserved in ethyl alcohol. Gross ob- 

 servations and drawings were made in water or water/ 

 glycerine solution, to and from which the specimens 



were moved slowly through series of solutions. Dis- 

 sected specimens were stained overnight in methyl 

 blue/lactic acid solution; detached appendages were 

 oriented, drawn (usually without cover slips), mounted 

 in glycerine under 9-mm diameter cover slips and 

 sealed with a heavy ring of nail-polish lacquer. Usually 



