﻿KESISTANCE OF ANIMALS TO MICRO-ORGANISMS. 7 



All the other animals under experiment and those used as controls are 

 alive 6/29/96. 



B. Inoculations with bacillus diphtheriae. 



Experiment III. 



Brotvn-Sequard experiment. — Five guinea-pigs were placed in bell-jars 

 of 14 litres capacity arranged in series. Experiment commenced Novem- 

 ber 7, 1896, and terminated December 19, 1896, when each of the 

 animals was inoculated with 1 mg. of a 34-hour old blood serum culture 

 of bacillus diphtheriae (attenuated). For the details of the experiment 

 see Table III. 



Table III. 



)ate, 1896. 



Hour. 



No. 1, 



640 g. 



No. 3, 



480 g. 



No. 3, 

 620 g. 



No. 4. 

 590 g. 



No. 5. 

 650 g. 



C.ft. 



air 



p'rh 



^ofCOs. 



Remarks. 



ov. 7 , . , , 



5.00 p. m. 















1st bell 

 jar. 



No. 1 is nearest the 

 pump. 



" 8.... 



11.30 a. m. 













11.0 







" 27.... 



5.00 p. m. 







+ 



+ 







4.06 





" 29 ... . 



12.00 " 







570 g. 



595 g. 









Nos. 3 and 4 dead. Re- 

 placed by fresh pigs. 



3C. 1 



3.00 " 



+ 













4.74 





' 9 



9.00 a. m. 



730 g. 















No. 1 dead. Replaced 

 by fresh pig. 



' 19 



4.15 p. m. 















L78 



Experiment stopped. 



' 19 



4.15 " 

















Inoculated with B. 

 diphtheriae. 







635 g. 



430 g. 



535 g. 



530 g. 



525 g. 







Present weight. 



No. 1 was found dead after 48 hours. 



2 



(( 



iC 



a 



i( 



39 



a 



3 



a 



iC 



« 



(( 



40 



cc 



4 



a 



it 



a 



u 



39 



(( 



5 



(C 



a 



a 



a 



47 



« 



Experiment IV. 



Carbonic acid gas. — Four guinea-pigs were placed under a 37 litre 

 bell-jar, November 17, 1896, and the experiment terminated December 

 19, 1896, when they were each inoculated with 1 mg. of a 24-hour old 

 blood serum culture of bacillus diphtheriae. The details of the experi- 

 ment are shown in Table IV. 



