﻿10 



RESISTANCE OF ANIMALS TO MICRO-ORGANISMS. 



I 



Inoculated with bacillus tuberculosis. 



5/17/97 No. 3 dead. Lungs and glands show masses of tubercules. 

 Liver and spleen smaller numbers. 



5/28/97 No. 4 killed. Lungs and glands show masses of tubercles. 

 Liver and spleen smaller numbers, but are very much congested. 



Table YL 



Date, 1896. 



Hour. 



No. 1, 

 200 g. 



No. 3, 

 330 gf. 



No. 3, 

 217 g. 



No. 4, 

 195 g. 



^of 

 CO2. 



Remarks. 



Dec. 28 



3.30 p. m. 















1897. 

















Jan. 15 



9.00 a. m. 









4- 





No. 4 dead. 



" 18.... 



3.30 p. m. 



+ 



+ 







3.47 



Air entering bell-jar. 



" 35 



10.00 a. m. 



272 g. 



277 g. 





360 g. 





Nos. 1 and 2 dead. Replaced by 

















fresh pigs. 



'* 29 



L30 p. m. 











14.9 



Air entering bell-jar. 



Feb. 4 



2.30 " 











0.4 



u u ti a. 



" 9 



3.00 " 











0.32 



U li u u 



" 23 



3.30 " 











7.01 



ii u u <t 



" 24 



3.00 " 













Experiment stopped. 



" 24 















Inoculated with anthrax vac. 



" 24 





252 g. 



236 g. 



248 g. 



285 g. 





Present weight. 



" 25 







+ 









No. 2 dead. Examination post mor- 

 tem negative. 



Mar. 4 















Inoculated with B. tuberculosis.' 



- 19 











+ 





No. 4 dead, bacilli found. 



" 30 









+ 







u 3 u u 



April 10... 





+ 











u 1 u u 



Summary of Results. 



In the staphylococcus and diphtheria inoculations the cultures used 

 appear to have been insufficiently attenuated to show any difference in 

 the effects produced upon the animals under experiment and the control 

 animals. It is, however, very doubtful whether cultures of these organ- 

 isms could be attenuated to such a degree as to still kill a weakened 

 animal and not kill a control, healthy animal. 



The anthrax vaccines used do not kill a healthy guinea-pig, but it was 

 expected that the animals might present sufficient lowering of the 

 vitality to become affected by the vaccines. This, however, was not the 

 case. The animals having failed to die from the effects of the anthrax 

 vaccines, they were then inoculated with an attenuated culture of tuber- 

 culosis. All the animals under experiment died much earlier than the 

 control animals. These results indicate a lowered vitality. Whether 

 this lowered vitality was brought about by the atmospheric conditions 

 under which they had lived, or whether it was brought about solely 

 through changes in their diet while under experiment, or whether both 

 these causes were active in producing the result, it is impossible to say. 

 The animals lost flesh and decreased in weight while under experiment. 

 It is not improbable that the loss in weight and the decrease in vitality 

 are both traceable to the same causes. 



