18 I. XAGAI : 



added iu the same proportion as before, for the chromogenic substauca P. 

 After the treated extracts were cool, the coloiu^s of the extracts were compai-ed 

 with those of the standard coloiu-s and recorded. The staudai'd colours were 

 prepai'ed iu the following manner. 



Twenty five cc • of quercetiu dissolved iu absolute alcohol, wer j reduced 

 wlthfive cc of concentrated hydi'ochloric acid and about 0.5 grams of magnesium 

 powder. 



Thus the relative value of the cluomogen content iu the material was 

 approximately determined. The result of a survey established the following 

 fact. 



The cln-omogeuic substance P cau be detected iu dillerent parts of plants, 

 i.e., the leaf, stem, shoot, rhizome, bark, wood, wliite petals, perigoue, seed 

 coat, mesocai"p, stigma etc. 



It occixrs quite iudepandently or in company ■n-ith the chromogenic sub- 

 stance F. Even iu the same plant, the distribution of the two chromogens is 

 quite chstinct in diflerent organs. 



Light seems to have no tlirect relation to the distribution of the chi-omo- 

 genic subs':a.nce P unlike the wise of the chromogenic substance i^ (flavones).' 

 For, the uudergroimd parts and the interior portions of the upper ground 

 tissues of many plants contain a considerable amount of the chromogenic 

 substance P. The bark of young twigs, the seed of imiujitnre seeds which 

 ultimately become brown, red or black when fiilly matiu-e, the young fraits 

 and berries of mauy of the cultivated fnüt trees are especially rich in the 

 clu-omogenic substance P. Thus, for example: 



1. Shtbata, K. Kagai, I. anil Kishida, JI , The Oocnrrenoc anil Physiological Significance of 

 Flavone Derivatives in Plants. Jour. Biol. Chem. 28 : 93, 191G. 



