A GENETICO-rHYSIOLOCilCAL STUDY ON THE FORMATION' ETC. 



; OO 



90 



80 



70 



GO 



SO 



¥0 



30 



A 



B 

 C 



10 



20 30 



^■0 

 t 



50 60 



Text-fig. 1. Gniphs showing the iltcom posit ion of aiathocyanin 

 by peroxiclnse. tee Table 1. 



shfiigbt line suggesting that the rate of decompositiou was fipproxiiuately 

 pi'oportional to the time of reaction, heuce the vahie of k calculated for the 

 imimolecular i-eaction iua-eased as time advanced. If, however, the value of 

 log. a — x are plotted against time, the cm-ves of B, C, and D do not form 

 sti'aight lines, while, the values of log. a—x are plotted against log. time, the 

 curves show more nearly the sti-aight lines. This seems to sho^\- that the 

 value of the enzyme was not constant. The active poiiiou of the enzyme de- 

 composed as the reaction proceeded. Two reactions, the decomposition of the 

 enzyme itself and that of authocyanin by the active portion of the enzyme in 

 the S}-stem seem to go on simultaneously, so that the rate of decomposition 

 of the latter cannot be regarded as a simple imi-molecular reaction. The 

 reaction may be uni-molecular only when the specific ratio of the enzyme to 

 the substrate in held and in which the rate of decompositiou of the enzyme 

 may be so slow that its value can be considered as nearly constant, while the 

 decomposition of the substance goes on with the constant rate. Hence we are 

 able to consider the value of a—x as the function of time. 



Similar observation was made with laccase. Ten cc of a 0.*) i^er cent 



