A GENETICO-rHYSIOLOGICAL STUDY ON THE FORMATION ETC. 5 



T<3 illustrate, tlio fi)llowing results will bo ineutioned. Ten gr<ims of tJie 

 fresh petals of scarlet Papavir Rh'jcus^ were exti'actecl with 150 ec of distilletl 

 water. The colonr of the exti-act wius deep red. This was takeu as 100, and 

 50, 25, auil 12') per cent S(Jutions were prepared. The psroxidase u.sed was 

 prepai'al fi'oiu the pi-essed saj) of hypx'utyls and rootlets of the .soy Ijean 

 see<miigs by precipitating with jilcrjhol. Five cc of the enzyme solution was 

 a<lded to fifty cc of a dilute hych-ogen peroxide solution (0.1 cc corresponded 

 to alx)ut 0.0003 g oxygen). The mixtures were jiut in Lirge test tubes of the 

 (L'ameter 4 ciu and they were kept in the water bath. The tempsratiu-e was 

 kept IjetwGeu 17.0 and 17.5 C. At thlferent intervals of time, five cc of the 

 mixture were pipetted out int<j a test tube and one cc of concenti-ated hydro- 

 chloric acid was added to stop the enzyme activity and to deepen the ciJonr. 

 The standard s<jlution was prepared in the sjxme manner immediately after 

 the enzyme was added. Each portion was then compai-ed witn tha standard 

 solution so prepai-ed by Dubosq's cfJiaimeter and the intensity of colom- of the 

 different poi-tions was expressed as jiercentages of the initial colour ^\hich was 

 taken as 100.- They gave the following result. 



T.U3LE 1. 



Decolorizntion of anthocynnin by peroxidase. 

 .\. Relative strength of .•mthocyanin = 100 

 l;. „ = 50.0 



C. ,. .. „ „ = 25.0 



D. „ = 125 



1. .\ix«raing to W'illstaitek an.l Wai. i Likbio. Xtxa. 412:231, 1910.) one of the xjigments 

 of a double puriJe-scarlet Tariity is mekocyunin which is a diglucoside of cyanidin. 



2. The initial colour corresponded with somewhere between Peach red to Scarlet in Ridoway's 

 Color St.amlard. 



