52 I. NAGAI : 



active \5er0xidase system tliau the rest of the pcntiou where the developmeut 

 of the anthocyauin pigmeut is inhibited, iu spite that the chi'omogeuic 

 substance cau be detected iu a shght extent. 



The above obsei-vation agrees with the findings of Keeble aud Aemstrong 

 (1912j' who have shown the paralleHsra extistiug iu the distribution of 

 anthocyauin and jieroxidase iu the corolla of PrivivJa sinensis and in others. 



When the seed coat is still gi-cen just previous to the formatiou of 

 anthocyaniu, a distinct red coloiu* develops at the poiiion of black patch b^' 

 treating the beau with hydi'ocliloric acid iu cold. It shows that the authocyaiiiu 

 pigmeut is ah"eady present iu a colourless state. 



Oxidation seemed to accelerate the development of anthocyauin in the 

 seed coat of the soy bean. Sh'ghtly coloured beans rapidly dee^jeued iu 

 colour if the pod was opened and exposed to ah'. Injmy also accelerated the 

 development of the pigment. Tlie portion near the injmy tecame pmple at 

 first ou exposm'e to air. Conversely the exclusion of air retarded the develop- 

 ment of the pigment. The sHghtly red-coloui-ed beans ceased to develop the 

 pra^Je pigmeut when kept in a closed chamber iu i\hich the air was 

 i-eplaced b}- hydi'ogen gas. Similar phenomena were observed iu rii^eniug 

 gi-ajies. In both cases, light had no influence. 



(b) The Cross : Blue Tinged Yellow x Brown aud 

 the Kec-iprocal. 



The colour of the seed coat of the parental plants are the following: 



" Tauislu." Yellow with a tinge of blue colour ^\hicli is most prominent 

 arouud the hilum, fading gi-adually further beyond. 



" Haiiro." Browu with a tinge of gi-ejish gi'een cok)ur. 



The i^ seeds produced ou the T\ plant were gi'eeu ^\•ith a pale blue 

 tiuge as in " Tauishi " (see Plate I). Iu F, solid blacks, aud non-tiuged 

 gi-eeus aud yellows were found, together with the parental t^^ie, by the 

 following uumbei"s. 



1. Keeble, F. ami Akmstbono, E. F., The Eole o£ Oxyiliuses in the Foriuntion of the .\ntho- 

 cyim rigmcnt of Plants. Jour. Gent. 2 : 277, 1912. 



2. Keeble, F. and Ai;meti;ong, E. F., Loc. cit. 



