72 I. NAGAI: 



the solvent yields an amoi-phons recltlisb brown pigment. Tlie pigment may 

 be precipitated from aqueons solution by le<id acetate. 



Consequently the gene / seems to inhibit the action of oxidizing agency 

 acting on the chi-omogenic substance. The alx)ve mentioned relation which 

 exists in buff and red seems to be analogous to that of the case of the 

 dominant white in Prininla sinensis. According to Keeble and Armstrong 

 (1912y, Keeble, Armstrong aud Jones (1913)- and Keeble and Miss 

 Pellow (1910)^ certain dominant wliites contain chromogen, which occurs in 

 the recessive white in au extremely slight amount and the inhibitory substance 

 which obscures the peroxidase reaction is present in the former. The buff 

 coloured seed coat of the Adzuki-beau can be considered somewhat analogous 

 to the dominant white in the flower of Primula and the white to the recessive 

 white. The colour of buii" aud white in the seed coat differ shghtly from 

 each other. The peroxidase reaction in the seed coat was also examined aud 

 an indication to the similar relation that was observed in the flower of 

 Primula sinensis was obtained. In the epidermis of the seed coat in which 

 the pigment is confined, the peroxidase reaction was extremely slight in the 

 unripe green seed of buff aud de?p bulf whereas in the white, very distinct 

 The observation was repeatedly made Avith the material taken at the different 

 stage of maturity. The section was placed under the cover glass with the 

 alcoholic solution of benzidine or alpha naphtol witli a dilute solution of 

 hydrogen fieroxide. In this manner, the direct oxidase has failed to be 

 detected in all cases. 



Tlie reddish bi-own in the soy beau (such as " Aka-nedzumime ") con-e- 

 spouds with red in the Adzuki-bean. The gene which modifies the redfhsh 

 •brown to bro^^•u iu the soy beau corresponds with the geue / in the other, 

 inasmuch as they suppress the formation of reddish-brown, oxidation product 

 of the chromogenic substance, though they differ in the manner toward the 

 formation of the chromogenic substance as already mentioned. The genetic 

 composition of the different self coloured typos in botli species can ba expressed 

 by the same designations iu the following manner : 



1, Keeble, F. nnl Akmsteong, E. F., Loc. cit. 



a. Keeble, F., Aemsteonq, E. F., an;l .Ioves. W. N., The Formation of the .Vnthooyan 

 Figments of Flnnts. 6. Proc. Roy. Soc. London, R 87 : 113, 1813. 



3. Keehle F. ami Fellow, C. White Flowered Varieties of Primula slnensus. Jour. Qeue- 

 tios. 1 : 1, lUlO. 



